Pipette 20 ml of endothelial cell growth medium to a t 75 flask.
Huvec primary cell line.
Promocell offers huvec isolated in standard endothelial cell growth medium.
Many of the endothelial processes are commonly studied in vitro using human umbilical vein endothelial cells huvec or large vessel and microvascular endothelial cells isolated from various body tissues.
Primary human umbilical vein endothelial cells huvec are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations such as macromolecule transport blood coagulation angiogenesis and fibrinolysis.
Passage normal huvec cells when culture has reached approximately 80 confluence.
Warm complete growth medium to 37 c prior to use with the cells.
Human umbilical vein endothelial cells huvec 10 donor pool 233 08.
They are guaranteed to express cd31 105 and survive in culture through 15 population doublings except huvec xl tm guaranteed through 5 pd.
All cells test negative for mycoplasma bacteria yeast and fungi.
Human umbilical vein endothelial cells huvec provide a classic model system to study many aspects of endothelial function and disease such as normal abnormal and tumor associated angiogenesis oxidative stress hypoxia and inflammation related pathways in endothelia under normal and pathological conditions cardiovascular related complications associated with various diseases mode of.
Product overview lonza s cryopreserved huvecs are shipped as frozen primaries.
Warm complete growth medium to 37 c prior to use with the cells.
Primary human umbilical vein endothelial cells huvec are isolated from human umbilical vein and cryopreserved at the end of the primary culture the cells are.
Take the endothelial cell growth medium from the refrigerator decontaminate the bottle with 70 alcohol in a sterile hood.
Passage normal huvec cells when culture has reached approximately 80 confluence.
Normal human umbilical vein endothelial cells huvec primary 309 09.
Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation.
Performance testedat least 16 population doublings when cultured in medium 200 supplemented with low serum growth supplement lsgs guarant.
Human umbilical vein endothelial cells huvec 10 donor pool.
Preparing cell culture flasks for culturing huvec.
Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation.
