Human Bronchial Epithelial Cell Line

The respiratory epithelia are responsible for the lubrication of the lungs the maintenance of humidity and the cleaning of the respiratory tract.

Human bronchial epithelial cell line.

Flick the tip of the conical tube with your finger to loosen the cell pellet. The 16hbe14o cell line was originally developed from human bronchial epithelium to study the cystic fibrosis transmembrane conductance cftr regulator cozens et al 1994 and retains many features of differentiated bronchial epithelial cells. Resuspend the cells in 2 ml of bronchial tracheal epithelial growth medium by gently pipetting the cells to break up the clumps. Count the cells with a hemocytometer or cell counter.

16hbe14o human bronchial epithelial cell line 16hbe14o human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of cftr. Primary human bronchial epithelial cells hbepc are isolated from the surface epithelium of human bronchi and stain positive for cytokeratin. Aspirate the supernatant from the tube without disturbing the cell pellet. Atcc normal human primary bronchial tracheal epithelial cells when grown in airway epithelial cell basal media supplemented with bronchial tracheal epithelial cell growth kit components provide an ideal cell system to propagate bronchial tracheal epithelial cells in serum free conditions.

Atcc primary cell solutions offers epithelial cells isolated from human breast bronchi trachea and small airways cornea prostate and kidneys. Calu 3 cells human bronchial epithelial cell line are originally derived from the subbronchial epithelium of a lung adenocarcinoma atcc manassas va and have been used as surrogate nasal epithelium due to their similar biophysical properties such as forming tight monolayers and also secreting mucus when cultured in an air liquid. These cells are ideally suited to support the study of many fields including inflammation cancer gene regulation cell matrix interactions and also have application in toxicology testing and drug screening development. 16hbe14o cells have a cobblestone appearance in culture form tight junctions and display.

The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology cytokeratin expression the. Further observation indicates distinct viral replication with a dynamic. Investigation of this viral infection in the human bronchial epithelial cell line 16hbe suggests that sars cov 2 can enter these cells through interaction between its membrane localized s protein with the angiotensin converting enzyme 2 molecule on the host cell membrane.